血管内皮细胞条件培养基促进胃癌细胞增殖和迁移

探讨血管内皮细胞培养基对胃癌细胞增殖和迁移的影响,分析血管内皮细胞调控胃癌细胞发生转移的机制。方法 设置对照组和共培养组,分别将正常培养基和人脐静脉血管内皮细胞HUVEC条件培养基作用于胃癌细胞HGC27,采用MTT法和划痕试验检测胃癌细胞HGC27的增殖活性和迁移能力。设置Control组、6 h组、12 h组和24 h组,以HUVEC的条件培养基作用于胃癌细胞6、12和24 h,Western blot检测EMT标志物和紧密连接蛋白的表达变化,激光共聚焦显微镜观察细胞骨架和紧密连接蛋白的分布变化。结果 MTT及划痕试验表明HUVEC条件培养基促进胃癌细胞HGC27的增殖和迁移。与Control组比较,间接共培养后胃癌细胞的形态呈间充质状改变,丝状伪足样凸起数量显著增加。 Western blot结果显示间接共培养后胃癌细胞上皮标志物E-cadherin表达水平逐渐下降,而间充质标志物N-cadherin和MMP-9逐渐增加,具有时序性变化规律。ZO-1和Occludin的表达也逐渐下降,细胞膜分布减少。结论 间接共培养下,血管内皮细胞通过上调胃癌细胞MMP-9,破坏紧密连接,促进胃癌细胞增殖和迁移     

Pediatric cerebral ganglioneuroblastoma

We describe a 4-year-old girl with acute onset headaches and transient blindness who underwent surgical intervention, chemotherapy, and radiotherapy for an intracranial mass. This mass was pathologically confirmed as a primary intracranial ganglioneuroblastoma, a rare finding in the pediatric population. The literature on pediatric primary intracranial ganglioneuroblastoma is reviewed.     

The effect of labor dance on perceived labor pain,birth satisfaction,and neonatal outcomes

ObjectiveThis research was conducted to determine the effects of labor dance on perceived birth pain, birth satisfaction, and neonatal outcomes.DesignThis is an experimental study. Data were collected under three groups during the active phase of labor: the dance practitioner midwife group (DPMG, comprising 40 pregnant women), the dance practitioner spouse/partner group (DPSG, comprising 40 pregnant women) and the control group (CG, comprising 80 pregnant women).SettingThis study was conducted between 1 April 2017 and 31 October 2017 in Turkey.ParticipantsThis study was administered on pregnant women volunteers with no risk during the active phase of labor.InterventionsDuring the active phase, pregnant women in DPMG danced with the midwife; pregnant women in DPSG, on the other hand, danced with their spouses/partners throughout the active phase. When vaginal dilatation reached 4 cm and 9 cm, labor pain was measured by employing the visual analog scale (VAS). In the postpartum phase, newborn babies’ first, fifth, and tenth minute Apgar scores and oxygen saturation levels were measured and registered. In the first hour after delivery, the Mackey Birth Satisfaction Scale was administered. CG, on the other hand, received only the routine procedures offered in the hospital.FindingsThe mean scores of VAS 1 and VAS 2 in DPSG and DPMG were lower than in CG. The fifth and tenth minute Apgar scores and the first, fifth, and tenth minute oxygen saturation levels of the newborns in the experimental groups, as well as the level of birth satisfaction, were significantly higher than in CG.Key conclusionsThe study showed a positive effect of labor dancing on the labor process.     

影响肝硬化腹水并发腹膜炎患者预后的危险因素分析

目的探讨影响肝硬化腹水并发腹膜炎（SBP）患者预后的危险因素。方法随机选择肝硬化腹水并发SBP患者115例，存活组60例，死亡组55例。统计年龄、性别、临床症状、肝硬化其他并发症、肝脏生化、腹水检查结果等18项指标，进行统计学分析。结果年龄、消化道出血、肝性脑病、肝肾综合征、休克、外周血白细胞数、凝血酶原活动度、血清总胆红素、血清白蛋白、血清钠、血清尿素氮、腹水白细胞数等与肝硬化腹水并发SBP患者预后有关（P〈0．05）。结论对于肝硬化腹水并发SBP患者，年龄越大、感染越重、合并肝硬化其他并发症、肝脏储备功能越差者，预后越不良。早期判断、预防、治疗这些危险因素，对改善预后有重要意义。     

中药对小白鼠骨骼肌切伤后再生影响的组织学观察

目的：探讨中药对骨骼肌切伤后再生的影响。方法：昆明种小鼠50只，制作骨骼肌纵行切伤模型，口服中药为实验组，未用中药为对照组。术后分别于第2、3、5、7、10天处死动物。组织切片，HE染色，观察骨骼肌再生的情况，以评价中药对损伤骨骼肌再生的影响。结果：实验组切口处肌卫星细胞激活；成肌细胞增殖、分化、融合以及肌管形成均快于对照组。结论：中药可以促进骨骼肌切伤后的再生。     

10万元以上医疗设备经济效益分析

根据我院近5年单价10万元以上医疗设备临床运行效益情况,对其设备整体效益进行分析研究,为医疗设备购置的合理性和可行性提供科学的依据,以便提高医疗设备运行效益的科学管理。